New Technology

Millisecond H/D Exchange

Many intrinsically disordered proteins possess transiently-structured elements.  These structural elements are difficult to detect by conventional methods.  To detect these elements with H/D exchange requires deuterium labeling and quenching on the millisecond time-scale.  To meet this need, we have developed a quench-flow system for H/D exchange. [Publication link]

Refrigerated Column Compartment for H/D Exchange Mass Spectrometry

To preserve the deuterium labeling during LC-MS, it is essential to hold the sample at 0 °C.  We developed a thermoelectrically-cooled column compartment for LC-MS.  A single twelve-port valve handles all steps of the analysis:  on-line digestion of the protein, desalting of the resultant peptides, and gradient elution.  The unit features integrated heat exchangers for efficient mobile phase cooling and independent control of the protease column temperature.  [Publication link]

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